![]() This helps reduce protein modifications and helps produce sharp band resolution. Bis-Tris chemistry provides a neutral (pH 7.0) environment during electrophoresis, which may result in better sample integrity and stability of the gel. Choose Bis-Tris gel chemistry when you have a low abundance of protein or when your downstream applications require high protein integrity, such as posttranslational modification analysis, mass spectrometry, or sequencing. For separation of a broad range of proteins, two chemistries, Bis-Tris and Tris-glycine, have been optimized for performance and long shelf life. SureCast reagents, other popular polyacrylamide reagentsįor denaturing gel electrophoresis or SDS PAGE there are many options available based on the application, and size of the protein of interest. Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only) Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell High capacity sample wells-load up to 60 µL per well.Special coating provides uniform polymerization rate for a more consistent handcast gel.Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage).Simple component assembly-using a single-motion load-and-lock mechanism.Unique tilt feature-minimize spillage and confidently load acrylamide solutions.Leak-free design-minimize the number of failed gels, waste less time.Low molecular weight proteins, western blotting, in-gel staining, and denaturing applications. High molecular weight proteins, Western blotting, mass spectrometry, post translationally modified proteins, and denaturing or native PAGE applications. Western blotting, in-gel staining, samples containing detergent and high salt, and denaturing or native PAGE applications. Western blotting, in-gel staining, mass spectrometry, post translationally modified proteins, dilute samples, and denaturing applications. ![]() Neutral pH for better separation, transfer, and analysis of high molecular weight proteins.īetter separation, transfer, and analysis of low molecular weight proteins. Traditional Laemmli-style gels with larger loading capacity-load up to 60 µL per well. ![]() Two buffer options: Choose MOPS buffer for higher range separations or MES buffer for lower range separations. Room temperature storage for up to 16 months. Load up to 60 µL per well with Bolt gels. Neutral pH helps prevent protein degradation for high sensitivity. NuPAGE Bis-Tris Gels, Bolt Bis-Tris Plus Gels ![]()
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